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atf4 immunofluorescence staining  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc atf4 immunofluorescence staining
    Atf4 Immunofluorescence Staining, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/immunofluorescence+antibody/us12600720-355-16-26
    Average 86 stars, based on 1 article reviews
    atf4 immunofluorescence staining - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Incubation:

    Article Title: Acute Shear Stress Induces TWIST-Mediated EndMT in Venous Endothelial Cells and Human Long Saphenous Veins
    Article Snippet: .. Cells were incubated in Immunofluorescence Blocking Buffer (Cell Signaling Technology, Danvers, MA, USA, 12411) for 30 min at room temperature, followed by overnight primary antibody incubation in Immunofluorescence Antibody Dilution Buffer (Cell Signaling Technology, 12378) at 4 °C and AlexaFluor fluorophore-conjugated secondary antibody for 1 h at room temperature. ..

    Article Title: Targeting SNAI1-Mediated Colorectal Cancer Chemoresistance and Stemness by Sphingosine Kinase 2 Inhibition.
    Article Snippet: CRC cells grown on coverslips were fixed in 100% methanol Articles © The authors | Journal compilation © World J Oncol and Elmer Press IncTM | www.wjon.org 747 for 10 min at room temperature and permeabilized with 0.1% Triton X-100 followed by incubation with blocking buffer (Cell Signaling Technology, Danvers, MA) for 1 h at room temperature. .. Next, the cells were incubated with mouse antiE-cadherin monoclonal antibody (1:100, Santa Cruz Biotechnology, Dallas, TX) diluted in immunofluorescence antibody dilution buffer (Cell Signaling Technology, Danvers, MA) overnight at 4 °C. .. The cells were then washed three times in 1 × phosphate-buffered saline (PBS) for 5 min each and were incubated with Alexa 488-conjugated anti-mouse secondary antibody (Cell Signaling Technology, Danvers, MA) for 1 h at room temperature.

    Article Title: Targeting SNAI1-Mediated Colorectal Cancer Chemoresistance and Stemness by Sphingosine Kinase 2 Inhibition
    Article Snippet: CRC cells grown on coverslips were fixed in 100% methanol for 10 min at room temperature and permeabilized with 0.1% Triton X-100 followed by incubation with blocking buffer (Cell Signaling Technology, Danvers, MA) for 1 h at room temperature. .. Next, the cells were incubated with mouse anti-E-cadherin monoclonal antibody (1:100, Santa Cruz Biotechnology, Dallas, TX) diluted in immunofluorescence antibody dilution buffer (Cell Signaling Technology, Danvers, MA) overnight at 4 °C. .. The cells were then washed three times in 1 × phosphate-buffered saline (PBS) for 5 min each and were incubated with Alexa 488-conjugated anti-mouse secondary antibody (Cell Signaling Technology, Danvers, MA) for 1 h at room temperature.

    Article Title: Spatial Transcriptomic Profiling of Human Saphenous Vein Exposed to Ex Vivo Arterial Haemodynamics-Implications for Coronary Artery Bypass Graft Patency and Vein Graft Disease.
    Article Snippet: .. Sections were then incubated in immunofluorescence blocking buffer (Cell Signalling Technology, Danvers, MA, USA, 12411) for 30 min at room temperature, followed by overnight primary antibody (Table 6) incubation in immunofluorescence antibody dilution buffer (Cell Signalling Technology, 12378) at 4 ◦C. ..

    Article Title: Acute Shear Stress Induces TWIST-Mediated EndMT in Venous Endothelial Cells and Human Long Saphenous Veins
    Article Snippet: .. Sections were then incubated in an Immunofluorescence Blocking Buffer (Cell Signalling Technology, 12411) for 30 min at room temperature, followed by overnight primary antibody incubation in an Immunofluorescence Antibody Dilution Buffer (Cell Signalling Technology, 12378) at 4 °C. ..

    Article Title: Acute Shear Stress Induces TWIST-Mediated EndMT in Venous Endothelial Cells and Human Long Saphenous Veins.
    Article Snippet: .. Sections were then incubated in an Immunofluorescence Blocking Buffer (Cell Signalling Technology, 12411) for 30 min at room temperature, followed by overnight primary antibody incubation in an Immunofluorescence Antibody Dilution Buffer (Cell Signalling Technology, 12378) at 4 ◦C. ..

    Article Title: Acute Shear Stress Induces TWIST-Mediated EndMT in Venous Endothelial Cells and Human Long Saphenous Veins.
    Article Snippet: .. Cells were incubated in Immunofluorescence Blocking Buffer (Cell Signaling Technology, Danvers, MA, USA, 12411) for 30 min at room temperature, followed by overnight primary antibody incubation in Immunofluorescence Antibody Dilution Buffer (Cell Signaling Technology, 12378) at 4 ◦C and AlexaFluor fluorophore-conjugated secondary antibody for 1 h at room temperature. ..

    Immunofluorescence:

    Article Title: Acute Shear Stress Induces TWIST-Mediated EndMT in Venous Endothelial Cells and Human Long Saphenous Veins
    Article Snippet: .. Cells were incubated in Immunofluorescence Blocking Buffer (Cell Signaling Technology, Danvers, MA, USA, 12411) for 30 min at room temperature, followed by overnight primary antibody incubation in Immunofluorescence Antibody Dilution Buffer (Cell Signaling Technology, 12378) at 4 °C and AlexaFluor fluorophore-conjugated secondary antibody for 1 h at room temperature. ..

    Article Title: Targeting SNAI1-Mediated Colorectal Cancer Chemoresistance and Stemness by Sphingosine Kinase 2 Inhibition.
    Article Snippet: CRC cells grown on coverslips were fixed in 100% methanol Articles © The authors | Journal compilation © World J Oncol and Elmer Press IncTM | www.wjon.org 747 for 10 min at room temperature and permeabilized with 0.1% Triton X-100 followed by incubation with blocking buffer (Cell Signaling Technology, Danvers, MA) for 1 h at room temperature. .. Next, the cells were incubated with mouse antiE-cadherin monoclonal antibody (1:100, Santa Cruz Biotechnology, Dallas, TX) diluted in immunofluorescence antibody dilution buffer (Cell Signaling Technology, Danvers, MA) overnight at 4 °C. .. The cells were then washed three times in 1 × phosphate-buffered saline (PBS) for 5 min each and were incubated with Alexa 488-conjugated anti-mouse secondary antibody (Cell Signaling Technology, Danvers, MA) for 1 h at room temperature.

    Article Title: Targeting SNAI1-Mediated Colorectal Cancer Chemoresistance and Stemness by Sphingosine Kinase 2 Inhibition
    Article Snippet: CRC cells grown on coverslips were fixed in 100% methanol for 10 min at room temperature and permeabilized with 0.1% Triton X-100 followed by incubation with blocking buffer (Cell Signaling Technology, Danvers, MA) for 1 h at room temperature. .. Next, the cells were incubated with mouse anti-E-cadherin monoclonal antibody (1:100, Santa Cruz Biotechnology, Dallas, TX) diluted in immunofluorescence antibody dilution buffer (Cell Signaling Technology, Danvers, MA) overnight at 4 °C. .. The cells were then washed three times in 1 × phosphate-buffered saline (PBS) for 5 min each and were incubated with Alexa 488-conjugated anti-mouse secondary antibody (Cell Signaling Technology, Danvers, MA) for 1 h at room temperature.

    Article Title: Spatial Transcriptomic Profiling of Human Saphenous Vein Exposed to Ex Vivo Arterial Haemodynamics-Implications for Coronary Artery Bypass Graft Patency and Vein Graft Disease.
    Article Snippet: .. Sections were then incubated in immunofluorescence blocking buffer (Cell Signalling Technology, Danvers, MA, USA, 12411) for 30 min at room temperature, followed by overnight primary antibody (Table 6) incubation in immunofluorescence antibody dilution buffer (Cell Signalling Technology, 12378) at 4 ◦C. ..

    Article Title: Defining the Antitumor Mechanism of Action of a Clinical-stage Compound as a Selective Degrader of the Nuclear Pore Complex
    Article Snippet: Cells were then blocked with Immunofluorescence Blocking Buffer (12411S, Cell Signaling Technology) for 1 hour. .. Primary antibodies were diluted in Immunofluorescence Antibody Dilution Buffer (12378, Cell Signaling Technology) and added to the cells. ..

    Article Title: Acute Shear Stress Induces TWIST-Mediated EndMT in Venous Endothelial Cells and Human Long Saphenous Veins
    Article Snippet: .. Sections were then incubated in an Immunofluorescence Blocking Buffer (Cell Signalling Technology, 12411) for 30 min at room temperature, followed by overnight primary antibody incubation in an Immunofluorescence Antibody Dilution Buffer (Cell Signalling Technology, 12378) at 4 °C. ..

    Article Title: Acute Shear Stress Induces TWIST-Mediated EndMT in Venous Endothelial Cells and Human Long Saphenous Veins.
    Article Snippet: .. Sections were then incubated in an Immunofluorescence Blocking Buffer (Cell Signalling Technology, 12411) for 30 min at room temperature, followed by overnight primary antibody incubation in an Immunofluorescence Antibody Dilution Buffer (Cell Signalling Technology, 12378) at 4 ◦C. ..

    Article Title: Acute Shear Stress Induces TWIST-Mediated EndMT in Venous Endothelial Cells and Human Long Saphenous Veins.
    Article Snippet: .. Cells were incubated in Immunofluorescence Blocking Buffer (Cell Signaling Technology, Danvers, MA, USA, 12411) for 30 min at room temperature, followed by overnight primary antibody incubation in Immunofluorescence Antibody Dilution Buffer (Cell Signaling Technology, 12378) at 4 ◦C and AlexaFluor fluorophore-conjugated secondary antibody for 1 h at room temperature. ..

    Blocking Assay:

    Article Title: Acute Shear Stress Induces TWIST-Mediated EndMT in Venous Endothelial Cells and Human Long Saphenous Veins
    Article Snippet: .. Cells were incubated in Immunofluorescence Blocking Buffer (Cell Signaling Technology, Danvers, MA, USA, 12411) for 30 min at room temperature, followed by overnight primary antibody incubation in Immunofluorescence Antibody Dilution Buffer (Cell Signaling Technology, 12378) at 4 °C and AlexaFluor fluorophore-conjugated secondary antibody for 1 h at room temperature. ..

    Article Title: Spatial Transcriptomic Profiling of Human Saphenous Vein Exposed to Ex Vivo Arterial Haemodynamics-Implications for Coronary Artery Bypass Graft Patency and Vein Graft Disease.
    Article Snippet: .. Sections were then incubated in immunofluorescence blocking buffer (Cell Signalling Technology, Danvers, MA, USA, 12411) for 30 min at room temperature, followed by overnight primary antibody (Table 6) incubation in immunofluorescence antibody dilution buffer (Cell Signalling Technology, 12378) at 4 ◦C. ..

    Article Title: Acute Shear Stress Induces TWIST-Mediated EndMT in Venous Endothelial Cells and Human Long Saphenous Veins
    Article Snippet: .. Sections were then incubated in an Immunofluorescence Blocking Buffer (Cell Signalling Technology, 12411) for 30 min at room temperature, followed by overnight primary antibody incubation in an Immunofluorescence Antibody Dilution Buffer (Cell Signalling Technology, 12378) at 4 °C. ..

    Article Title: Acute Shear Stress Induces TWIST-Mediated EndMT in Venous Endothelial Cells and Human Long Saphenous Veins.
    Article Snippet: .. Sections were then incubated in an Immunofluorescence Blocking Buffer (Cell Signalling Technology, 12411) for 30 min at room temperature, followed by overnight primary antibody incubation in an Immunofluorescence Antibody Dilution Buffer (Cell Signalling Technology, 12378) at 4 ◦C. ..

    Article Title: Acute Shear Stress Induces TWIST-Mediated EndMT in Venous Endothelial Cells and Human Long Saphenous Veins.
    Article Snippet: .. Cells were incubated in Immunofluorescence Blocking Buffer (Cell Signaling Technology, Danvers, MA, USA, 12411) for 30 min at room temperature, followed by overnight primary antibody incubation in Immunofluorescence Antibody Dilution Buffer (Cell Signaling Technology, 12378) at 4 ◦C and AlexaFluor fluorophore-conjugated secondary antibody for 1 h at room temperature. ..



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    Image Search Results


    Bilayer hydrogel orchestrates inflammatory cell dynamics during the early inflammation phase of diabetic wound healing. a Experimental timeline for assay of early neutrophil recruitment. b Immunohistochemical staining for Ly-6G in wounds at 8 h, 1 d and 3 d after injury. Diabetic wounds were treated with SP/IL-10@Bilayer, SP@Bilayer, IL-10@Bilayer, and saline solution (Model), respectively. Healthy mice treated with saline solution were set as Normal. c Quantitative analysis of Ly-6G + cells in each group. d Relative expression of CXCL-1 on day 1. e Relative expression of MCP-1 on day 1. f Experimental timeline for assay of M1 macrophage infiltration. g Immunofluorescence staining for iNOS in wounds on days 1, 3 and 6 after injury. h Quantitative analysis of iNOS + cells in each group. i-k Relative expressions of macrophage-associated pro-inflammatory cytokines including TNF-α, IL-1β and IL-6 on day 3. l Schematic illustrating the dynamic modulation of inflammatory cells during the early inflammation phase of diabetic wounds by SP/IL-10@Bilayer. All data were generated from at least three independent experiments and presented as the means ± standard deviation. Statistical analysis was performed by one-way ANOVA. # means significant difference compared to the normal group. #p < 0.05, ##p < 0.01 and ###p < 0.001; ∗ means significant difference compared to the model group. ∗p < 0.05; & means significant difference compared to SP/IL-10@Bilayer. & p < 0.05 and && p < 0.01.

    Journal: Bioactive Materials

    Article Title: Skin-mimetic bilayer hydrogel normalizes diabetic wound healing by orchestrating inflammatory cell dynamics: An early intervention strategy

    doi: 10.1016/j.bioactmat.2026.02.025

    Figure Lengend Snippet: Bilayer hydrogel orchestrates inflammatory cell dynamics during the early inflammation phase of diabetic wound healing. a Experimental timeline for assay of early neutrophil recruitment. b Immunohistochemical staining for Ly-6G in wounds at 8 h, 1 d and 3 d after injury. Diabetic wounds were treated with SP/IL-10@Bilayer, SP@Bilayer, IL-10@Bilayer, and saline solution (Model), respectively. Healthy mice treated with saline solution were set as Normal. c Quantitative analysis of Ly-6G + cells in each group. d Relative expression of CXCL-1 on day 1. e Relative expression of MCP-1 on day 1. f Experimental timeline for assay of M1 macrophage infiltration. g Immunofluorescence staining for iNOS in wounds on days 1, 3 and 6 after injury. h Quantitative analysis of iNOS + cells in each group. i-k Relative expressions of macrophage-associated pro-inflammatory cytokines including TNF-α, IL-1β and IL-6 on day 3. l Schematic illustrating the dynamic modulation of inflammatory cells during the early inflammation phase of diabetic wounds by SP/IL-10@Bilayer. All data were generated from at least three independent experiments and presented as the means ± standard deviation. Statistical analysis was performed by one-way ANOVA. # means significant difference compared to the normal group. #p < 0.05, ##p < 0.01 and ###p < 0.001; ∗ means significant difference compared to the model group. ∗p < 0.05; & means significant difference compared to SP/IL-10@Bilayer. & p < 0.05 and && p < 0.01.

    Article Snippet: The infiltration of pro-inflammatory (M1) macrophages and polarization of M2c macrophages were analyzed by immunofluorescence staining using antibodies against iNOS (Servicebio, GB11119) and CD163 (Servicebio, GB14027), respectively.

    Techniques: Immunohistochemical staining, Staining, Saline, Expressing, Immunofluorescence, Generated, Standard Deviation

    Bilayer hydrogel modulates M2c macrophage polarization in the later healing phase of diabetic wound. a Experimental timeline for assay of M2c macrophage polarization. b Immunofluorescence staining for CD163 in wounds on days 3, 6, 9 and 12 after injury. c Quantitative analysis of CD163 + cells in each group. d-f Relative expression of MerTK, IL-10, and TGF-β1 on day 6. g Schematic illustration of M2c macrophage polarization regulated by SP/IL-10@Bilayer and its contribution to inflammation resolution. All data were generated from three independent experiments and presented as the means ± standard deviation. Statistical analysis was performed by one-way ANOVA. # means significant difference compared to the normal group. #p < 0.05 and ##p < 0.01; ∗ means significant difference compared to model group. ∗p < 0.05, ∗∗p < 0.01 and ∗∗∗p < 0.001; & means significant difference compared to SP/IL-10@Bilayer group. & p < 0.05.

    Journal: Bioactive Materials

    Article Title: Skin-mimetic bilayer hydrogel normalizes diabetic wound healing by orchestrating inflammatory cell dynamics: An early intervention strategy

    doi: 10.1016/j.bioactmat.2026.02.025

    Figure Lengend Snippet: Bilayer hydrogel modulates M2c macrophage polarization in the later healing phase of diabetic wound. a Experimental timeline for assay of M2c macrophage polarization. b Immunofluorescence staining for CD163 in wounds on days 3, 6, 9 and 12 after injury. c Quantitative analysis of CD163 + cells in each group. d-f Relative expression of MerTK, IL-10, and TGF-β1 on day 6. g Schematic illustration of M2c macrophage polarization regulated by SP/IL-10@Bilayer and its contribution to inflammation resolution. All data were generated from three independent experiments and presented as the means ± standard deviation. Statistical analysis was performed by one-way ANOVA. # means significant difference compared to the normal group. #p < 0.05 and ##p < 0.01; ∗ means significant difference compared to model group. ∗p < 0.05, ∗∗p < 0.01 and ∗∗∗p < 0.001; & means significant difference compared to SP/IL-10@Bilayer group. & p < 0.05.

    Article Snippet: The infiltration of pro-inflammatory (M1) macrophages and polarization of M2c macrophages were analyzed by immunofluorescence staining using antibodies against iNOS (Servicebio, GB11119) and CD163 (Servicebio, GB14027), respectively.

    Techniques: Immunofluorescence, Staining, Expressing, Generated, Standard Deviation